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pmt 025 vector  (Addgene inc)


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    Addgene inc pmt 025 vector
    Pmt 025 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmt+025+vector/pMT_025+(Plasmid+%23158579)/pm41423452-225-13-17
    Average 93 stars, based on 7 article reviews
    pmt 025 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Deep Mutational Scanning of FDX1 Identifies Key Structural Determinants of Lipoylation and Cuproptosis
    Article Snippet: A library of FDX1 full-length ORF variant oligonucleotides was synthesized at Twist BioScience. .. The fragment library was digested with NheI and BamHI, and ligated into the pMT_025 vector (available from Addgene). .. The fragment library was digested with NheI and BamHI, and ligated into the pMT_025 vector (available from Addgene).

    other:

    Article Title: Deep Mutational Scanning of FDX1 Identifies Key Structural Determinants of Lipoylation and Cuproptosis.
    Article Snippet: A library of FDX1 full-length ORF variant oligonucleotides was synthesized at Twist BioScience.

    Ligation:

    Article Title: A comprehensive map of missense trafficking variants in rhodopsin and their response to pharmacologic correction
    Article Snippet: .. The ORF was flanked with adapters suitable for restriction digestion and ligation into NheI and BamHI sites of pMT_025 vector (Addgene #158579). .. PCRs were performed using high-fidelity polymerase to ensure the variant was incorporated into the newly synthesized DNA according to manufacturer’s instructions (Twist Bioscience).



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    pmt 025 vector  (Addgene inc)
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    Addgene inc pmt 025 vector
    Pmt 025 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmt+025+vector/pMT_025+(Plasmid+%23158579)/pm41423452-225-13-17
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    pmt025 backbone vector  (Addgene inc)
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    A Volcano plot shows the CRISPR/Cas9 FDX1 loss-of-function viability outcome in 1373 cell lines correlated with the loss of function of all other tested genes. Plotted are the correlation values and the p -value for the 17,915 genes (see Methods for details). Possible FDX1 upstream regulators are colored orange. B Schematic of an alternative upstream regulation of FDX1. C Immunoprecipitation (IP) with anti-Strep-Tag II (STII) of C-terminally STII-tagged DLD or FDXR stably expressed in HEK293T cells. Indicated FDX1 constructs are transiently transfected. <t>pMT025</t> is the same expression vector used for the DMS screen, and “stop” indicates a stop codon (i.e., no tag). ORF, open reading frame. Endo, endogenous. D In vitro FDX1- and LIAS-mediated lipoylation activity assay that compare FDXR and DLD as the upstream reducing source and FDXR alone as control. Data is presented as mean ± SD of three replicates. E − G CRISPR/Cas12a mediated DLD KO in JHH7 ( E ) and in HEK293T ( F ), and FDXR KO in JHH7 ( G ). The lysates were immunoblotted with the indicated antibodies. Green arrows highlight changes in levels of key proteins of interest. H Top, HEK293T overexpressing the indicated constructs were assayed for viability after 2 days of ES-Cu treatment. Bottom, immunoblots of cell lines shown above. Viability data is presented as mean ± SEM of five biological replicates. I Schematic depicting our model: during homeostasis, FDX1 uses its negative charges on its third alpha helix to facilitate critical downstream steps including lipoylation and Cu reduction in ES (left panel). By flipping the charges of those residues (right panel), an inhibitory protein binds mutant FDX1, preventing it from receiving electrons from DLD, FDXR, and/or other reducing sources. This results in a functionally inactive FDX1.
    Pmt025 Backbone Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmt+025+vector/pMT_025+(Plasmid+%23158579)/pmc12855868-251-8-11
    Average 93 stars, based on 1 article reviews
    pmt025 backbone vector - by Bioz Stars, 2026-09
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    pmt 025 lentiviral expression vector  (Addgene inc)
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    Addgene inc pmt 025 lentiviral expression vector
    A Volcano plot shows the CRISPR/Cas9 FDX1 loss-of-function viability outcome in 1373 cell lines correlated with the loss of function of all other tested genes. Plotted are the correlation values and the p -value for the 17,915 genes (see Methods for details). Possible FDX1 upstream regulators are colored orange. B Schematic of an alternative upstream regulation of FDX1. C Immunoprecipitation (IP) with anti-Strep-Tag II (STII) of C-terminally STII-tagged DLD or FDXR stably expressed in HEK293T cells. Indicated FDX1 constructs are transiently transfected. <t>pMT025</t> is the same expression vector used for the DMS screen, and “stop” indicates a stop codon (i.e., no tag). ORF, open reading frame. Endo, endogenous. D In vitro FDX1- and LIAS-mediated lipoylation activity assay that compare FDXR and DLD as the upstream reducing source and FDXR alone as control. Data is presented as mean ± SD of three replicates. E − G CRISPR/Cas12a mediated DLD KO in JHH7 ( E ) and in HEK293T ( F ), and FDXR KO in JHH7 ( G ). The lysates were immunoblotted with the indicated antibodies. Green arrows highlight changes in levels of key proteins of interest. H Top, HEK293T overexpressing the indicated constructs were assayed for viability after 2 days of ES-Cu treatment. Bottom, immunoblots of cell lines shown above. Viability data is presented as mean ± SEM of five biological replicates. I Schematic depicting our model: during homeostasis, FDX1 uses its negative charges on its third alpha helix to facilitate critical downstream steps including lipoylation and Cu reduction in ES (left panel). By flipping the charges of those residues (right panel), an inhibitory protein binds mutant FDX1, preventing it from receiving electrons from DLD, FDXR, and/or other reducing sources. This results in a functionally inactive FDX1.
    Pmt 025 Lentiviral Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmt+025+vector/pMT_025+(Plasmid+%23158579)/bio_rxiv__2025__01__31__635952-52-19-23
    Average 93 stars, based on 1 article reviews
    pmt 025 lentiviral expression vector - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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    A Volcano plot shows the CRISPR/Cas9 FDX1 loss-of-function viability outcome in 1373 cell lines correlated with the loss of function of all other tested genes. Plotted are the correlation values and the p -value for the 17,915 genes (see Methods for details). Possible FDX1 upstream regulators are colored orange. B Schematic of an alternative upstream regulation of FDX1. C Immunoprecipitation (IP) with anti-Strep-Tag II (STII) of C-terminally STII-tagged DLD or FDXR stably expressed in HEK293T cells. Indicated FDX1 constructs are transiently transfected. pMT025 is the same expression vector used for the DMS screen, and “stop” indicates a stop codon (i.e., no tag). ORF, open reading frame. Endo, endogenous. D In vitro FDX1- and LIAS-mediated lipoylation activity assay that compare FDXR and DLD as the upstream reducing source and FDXR alone as control. Data is presented as mean ± SD of three replicates. E − G CRISPR/Cas12a mediated DLD KO in JHH7 ( E ) and in HEK293T ( F ), and FDXR KO in JHH7 ( G ). The lysates were immunoblotted with the indicated antibodies. Green arrows highlight changes in levels of key proteins of interest. H Top, HEK293T overexpressing the indicated constructs were assayed for viability after 2 days of ES-Cu treatment. Bottom, immunoblots of cell lines shown above. Viability data is presented as mean ± SEM of five biological replicates. I Schematic depicting our model: during homeostasis, FDX1 uses its negative charges on its third alpha helix to facilitate critical downstream steps including lipoylation and Cu reduction in ES (left panel). By flipping the charges of those residues (right panel), an inhibitory protein binds mutant FDX1, preventing it from receiving electrons from DLD, FDXR, and/or other reducing sources. This results in a functionally inactive FDX1.

    Journal: Nature Communications

    Article Title: Deep Mutational Scanning of FDX1 Identifies Key Structural Determinants of Lipoylation and Cuproptosis

    doi: 10.1038/s41467-025-67869-0

    Figure Lengend Snippet: A Volcano plot shows the CRISPR/Cas9 FDX1 loss-of-function viability outcome in 1373 cell lines correlated with the loss of function of all other tested genes. Plotted are the correlation values and the p -value for the 17,915 genes (see Methods for details). Possible FDX1 upstream regulators are colored orange. B Schematic of an alternative upstream regulation of FDX1. C Immunoprecipitation (IP) with anti-Strep-Tag II (STII) of C-terminally STII-tagged DLD or FDXR stably expressed in HEK293T cells. Indicated FDX1 constructs are transiently transfected. pMT025 is the same expression vector used for the DMS screen, and “stop” indicates a stop codon (i.e., no tag). ORF, open reading frame. Endo, endogenous. D In vitro FDX1- and LIAS-mediated lipoylation activity assay that compare FDXR and DLD as the upstream reducing source and FDXR alone as control. Data is presented as mean ± SD of three replicates. E − G CRISPR/Cas12a mediated DLD KO in JHH7 ( E ) and in HEK293T ( F ), and FDXR KO in JHH7 ( G ). The lysates were immunoblotted with the indicated antibodies. Green arrows highlight changes in levels of key proteins of interest. H Top, HEK293T overexpressing the indicated constructs were assayed for viability after 2 days of ES-Cu treatment. Bottom, immunoblots of cell lines shown above. Viability data is presented as mean ± SEM of five biological replicates. I Schematic depicting our model: during homeostasis, FDX1 uses its negative charges on its third alpha helix to facilitate critical downstream steps including lipoylation and Cu reduction in ES (left panel). By flipping the charges of those residues (right panel), an inhibitory protein binds mutant FDX1, preventing it from receiving electrons from DLD, FDXR, and/or other reducing sources. This results in a functionally inactive FDX1.

    Article Snippet: Full length FDX1 (NM_004109.5) was cloned into the pMT025 backbone vector (Addgene plasmid #158579) previously described , .

    Techniques: CRISPR, Immunoprecipitation, Strep-tag, Stable Transfection, Construct, Transfection, Expressing, Plasmid Preparation, In Vitro, Activity Assay, Control, Western Blot, Mutagenesis